INTRODUCTION TO FERMENTATION – Session in English
Biotechnologies
Objectifs
Introduce the fundamentals of microbial growth as a basis for understanding industrial fermentation technologies.
Provide hands-on training in conducting fermentation processes at the laboratory scale..
Participants should have prior knowledge of aseptic practices and a sufficient mathematical background (including logarithmic functions).
Contenu pédagogique
THEORY
Biological material
- Microbial strains: bacteria, yeasts, molds
- Nutrition and growth
Operation of fermenters
Kinetics
- Biomass production
- Metabolite production
- Substrate consumption
Production curves and calculations
- Analysis of production curves
- Calculation of representative culture parameters
- Yield and productivity calculations
Industrial applications of fermentation
Industrial fermenters and maintenance
PRACTICAL WORK
- Operation of laboratory fermenters
- Monitoring a culture in a fermenter: biomass and/or metabolite production
- Use of biological material: bacteria and/or yeast
- Physico-chemical monitoring: pH, temperature, oxygen…
- Microbiological monitoring: biomass estimation
- Biochemical monitoring: substrate/metabolite follow-up
- Case studies of common process malfunctions